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6. Centrifuge at 3500g for 10 min at room temperature, then transfer the aqueous phase into 50-mL glass centrifuge tubes. 7. Add 2.5 volumes of 95% ethanol; incubate at 20rC for at least 1 h. Centrifuge at 15,000g, 4rC for 20 min. Wash pellet with 1 mL of 70% ethanol and air-dry. 8. Resuspend DNA pellet in TE (see Note 18). 3.2.3.

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